ABSTRACT
Objective To establish a novel liver and lung metastatic tumor model in nude mice by splenic inoculation of hilar cholangiocarcinoma cell line.Methods Human hilar cholangiocarcinoma cell line(FPH-0201) cells was established and cultured with a fresh human hilar cholangiocarcinoma specimen.The spleen of 7 nude mice was injected with 0.1ml of hilar cholangiocarcinoma cell suspension(1.5?10~7 cell).The tumor appearing time,tumor local and metastatic formation rate,histological,morphological and ultrastructure of metastatic tumor cell were observed.The sub-cell line was obtained by cutture of metastatic specimen,and sub-cell line cells were again injected into the lower pole of the spleen and metastatic tumor formation rate was observed again.Results The overall local tumor formation rate in spleen was 100%,and metastatic tumor formation rate was 14.3%.The sub-cell line cells that were re-inoculated in mice spleen,had a metastatic tumor formation rate of 100%.Morphological observation of metastatic tumor showed the common features of cholangiocarcinoma.Chromosome number showed a variation of 18~44.Conclusions The hilar cholagiocarcinoma metastatic tumor model that was established in this laboratomy had characteristics compatible with malignant tumor and had all the biological characteristics of human hilar cholagiocarcinoma.